Fig. 6

Induction of pro-inflammatory cytokines in Ali-cultured immortalized STEC line and primary STECs in response to Toll-like receptor (TLR) agonist stimulation. Immortalized STEC line and primary STECs were cultured under Ali conditions. At D14, the cells were treated with TLR agonists for 24 h. The cellular supernatants were collected, and cytokine analysis was performed with TNFα and IL-1β ELISA kits. Data are presented as the ratios of cytokine expression by treated cells relative to expression by control cells in immortalized or primary cells, and given as the mean +/− SD of three experiments, each done in triplicate (n = 9). (*P < 0.05, **P < 0.01)