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Fig. 2 | BMC Cell Biology

Fig. 2

From: mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells

Fig. 2

Western blot analysis of anethole on adipogenic differentiation in hMSCs. a hMSCs were pretreated with 50 μM anethole and incubated in adipogenic media for 4 weeks. Once a week, whole cell pellets were lysed in lysis buffer and immnoblotted for p-mTOR, mTOR, p-p70S6K, p70S6K, PPAR-γ, p-Akt, Akt, p-AMPKand AMPK. b The phosphorylated expression of each protein was caculated by dividing the total form of corresponding protein, and was normalized by β-actin. The relative folds were measured and calculated using Image J. Each experiment was repeated three times and the values are presented as mean ± S.D. **p < 0.05, and ***p < 0.001

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