Fig. 3
From: mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells
Effect of anethole on excessive ROS generation in hMSCs. a hMSCs were exposed at 2 mM H2O2 for 30 min and incubated for 2 days in presence or absence of 50 μM anethole. ROS was measured by staining the cells with DCFDA cellular ROS detection assay kit according to the manufacturer’s instructions. After staining, cells were strained briefly and analyzed using Accuri-C6. b ROS generation was also observed under a fluorescence microscope at 200× magnification after same treatment previously described. c Whole cell pellets underwent western blot analysis for p-mTOR and p-AMPK. d Each protein expression was exhibited in the same manner as described in Fig. 3b. Each experiment was repeated three times and the values are presented as mean ± S.D. **p < 0.05, and ***p < 0.001