Fig. 1

Mouse small intestinal epithelial cells divide and maintain morphology with passage and express epithelial cell markers when cultured under modified conditional reprogramming conditions. a Representative phase contrast images of IEC monolayer colonies 1 day post-plating and 4 days after post-plating (passage 12). Dashed circle indicates colonies. Images were taken at 10× magnification. Scale bar: 100 μm. b Representative image of colony with higher magnification (20×) c Representative flow cytometry plot of EpCAM expression in IEC monolayers. Data represents IEC monolayers from 3 individual mice and diverse passages. d Representative phase contrast images of IEC monolayers colonies at early, middle and later passages. e Representative fluorescence microscopy images of IEC monolayers stained with tight junction markers E-cadherin and ZO-1