Fig. 2

Small intestinal IEC monolayers from WT, CFTR ∆F508 and Apc^Min/+ mice maintain presence of mutant allele across passages. a Genomic DNA isolated from IEC monolayers derived from wild-type (WT) or cystic fibrosis (CFTR ∆F508) mouse models amplified by CFTR primers. WT IEC monolayers and irradiated 3T3-J2 feeders contain the normal length CFTR gene and CFTR ∆F508 IEC monolayers contains the truncated CFTR gene. b Genomic DNA isolated from IEC monolayers derived from WT or intestinal cancer (Apc^Min/+) mouse models. IEC monolayers from Apc^Min/+ mouse model contains the mutant Apc gene. n = 3 individual animals in each genetic mouse model