Fig. 3From: Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analysesMouse CFTR ∆F508 IEC monolayers retains CFTR dysfunction in intestinal epithelium. a Representative images of WT and CFTR ∆F508 IEC monolayer derived spheroids grown in matrigel and treated with forskolin (Fsk) to induce spheroid swelling. Images were taken at 10× magnification. Scale bar: 100 μm. White dashed line: representation of spheroid diameter measurement. b Quantification of WT and CFTR ∆F508 IEC monolayers swelling in response to CFTR activation (Forskolin, Fsk) and inhibition (CFTRinh-172). *p < 0.05, n = 3 individual animals in each treatment groupBack to article page