Fig. 5

Mouse Apc^Min/+ tumor IEC monolayers retain functional characteristics of intestinal epithelium. a Representative images of WT and Apc^Min/+ tumor IEC monolayers colonies growing on a feeder layer at passage 0. b Quantification of WT and Apc^Min/+ tumor IEC monolayer colony growth between days 2 and 3 post-plating. c Representative images of WT and Apc^Min/+ tumor IEC monolayer derived spheroids grown in matrigel. d Quantification of WT and Apc^Min/+ tumor IEC monolayer derived spheroid growth based on spheroid diameter on day 5 and day 7 compared to day 3. *p < 0.05, n = 3 individual animals in each genetic mouse model