Fig. 2From: Novel roles for scleraxis in regulating adult tenocyte functionGC bias apparent in reads mapping to scleraxis (Scx) mRNA. Shown are reads from a representative scramble control mapped to the equine Scx mRNA (NM_001105150.1). GC content (blue line) of the reference sequence is shown relative to the AT content (green line). The majority of the reads mapped to the 5’ UTR, which has a GC content of approximately 55%. Few reads mapped to the coding sequence (CDS; ~ 72% GC) and those that did are preferentially located in non-GC biased regions. There were no reads that corresponded to the 3′ end of the Scx CDS, which exhibited the highest GC content (approximately 80%)Back to article page