Fig. 3
From: Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus
Expression of LPS-induced factors in exfoliative sulcular keratinocytes. a Immunocytochemical detection of ICAM-1, TLR-4, and TLR-2 expression (red) in sulcular epithelial cells. The nucleus was stained with Hoechst 33342 (blue). Bar = 50 μm. Graphs show the quantitative analysis of the percentage of positive cells. Mean number of the percentage of each positive cells ± SD from five independent studies. *Significantly different (Student’s t-test) at P < 0.05 compared with exfoliative cells from free gingiva. b Western blotting analysis of ICAM-1, TRL-4, and TLR-2 expression in exfoliative cells from gingival sulcus and free gingiva. β-actin (ACTB) was used as a loading control. Band densities were presented as antibodies’ expression fold-increases (normalized to ACTB) and compared with the results for exfoliative cells from free gingiva. Quantification results are shown below the corresponding blots. All experiments were performed in quadruplicate