Fig. 7
From: Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus
Induction of autophagy is dependent on TLR4 expression in PgLPS-stimulated HaCaT cells. HaCaT cells were stimulated for 24 hours in the absence (control, Ctr) or presence of PgLPS (10 μg/ml) with or without 3-MA (10 mM) or PMB (100 μg/ml). a Representative images of immunocytochemical expression of TLR-4 (Red) in HaCaT cells. Nuclei were stained with Hoechst 33342 (blue). Bar = 25 μm. b Western blotting analysis of TLR-4. β-actin (ACTB) was used as a loading control. Band densities were presened as antibodies’ expression fold-increases (normalized to ACTB) and compared with the results for untreated cells (Ctr). Quantification results are shown below the corresponding blots. All experiments were performed in quadruplicate