Fig. 5
From: Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32
C-terminal lysines modulate Cx32 turnover. a Cells expressing Myc/6xHis tagged WTCx32, 5RCx32, or 5QCx32 were pulsed with 35-S-Met/Cys for 5 min and lysed (0) or chased 1.5 h in complete medium (1.5). Cx32-Myc/His was isolated by affinity purification and processed for Western blotting. 35S-Met/Cys incorporation into newly synthesized Cx32-Myc/His was detected by phosphorimaging and the blots were subsequently probed with anti-Myc antibody to detect Cx32-Myc/His. b Densitometric quantification of 35-S Cx32 remaining after 1.5 h. 35-S was quantified and normalized to total Cx32-Myc amount (*p < 0.05, Student’s T-test, N = 3)