Fig. 6
From: Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32
C-terminal lysine mutants form functional gap junctions. a Dual whole-cell recordings showed no significant differences in junctional conductance (Gj) between WT, 5R, or 5Q cell pairs. Mean Junctional conductances for pIRES2EGFP (I), WT, 5R, and 5Q Cx32 are plotted. b Cells in experiments plotted here were transfected with 3-fold more DNA than in A; no influence on Gj activity is observed. c Representative average normalized Gj-Vj relations with 5R (left) and 5Q (right) are compared with Cx32WT channels. The average normalized instantaneous (open triangles) and steady-state (filled squares) junctional conductance (Gj) at each Vj were calculated from current traces and fit as described in the Materials and Methods. Solid lines represent fits to Cx32-5R or Cx32-5Q; broken lines represent fits to data for Cx32WT homotypic cell-cell channels