Fig. 1

Reduced expression of PIAS1 after ischemia/reperfusion in mouse myocardium and H9C2 cells. a H&E staining of representative mouse myocardial sections under different treatments. (i) Sham-operated, (ii) 2 h after I/R, (iii) 4 h after I/R, and (iv) 6 h after I/R. Scale bars, 100 μm. b RT-PCR analysis of selected SUMO E3 ligases in mouse myocardium after I/R (n = 3). c IHC analysis of PIAS1 expression in representative mouse myocardial sections under different treatments. (i) Sham-operated, (ii) 2 h after I/R, (iii) 4 h after I/R, and (iv) 6 h after I/R. Scale bars, 100 μm. d Quantification of PIAS1-positive cells (arrows in c) in mouse myocardial sections of different groups (n = 5). e IHC analysis of PIAS1 expression in human cardiac tissues from patients under different surgeries. Scale bars, 100 μm. f Western blot analysis of PIAS1 proteins in H9C2 cells under normoxic condition (Sham) or hypoxia and reoxygenation (H/R) treatments. GAPDH was set as loading control. g Quantification of the densitometry of the western blot band in F (n = 3). Experiments in a, b, e and f were performed three times, and experiments in c and d were performed five times. Data presented are means ± SD, ^*P < 0.05 vs. sham group, ^##P < 0.01 vs. I/R 2 h group, and ^$$P < 0.01 vs. I/R 4 h group