Skip to main content
Fig. 5 | BMC Cell Biology

Fig. 5

From: PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPARγ SUMOylation

Fig. 5

Ectopic expression of PIAS1 alleviates IRI via PPARy SUMOylation. a H9C2 cells were transfected with PIAS1 plasmids as indicated; then, cells were cultured under normoxic or H/R conditions. Cell lysates were immunoprecipitated with anti-PPARγ antibody, followed by blotting with anti-SUMO1 or anti-PPARγ antibody. b Quantification of the densitometry of the SUMO-PPARγ band in A (n = 3). c TUNEL staining of apoptotic H9C2 cells transfected with vector or PIAS1 under normoxic condition or H/R treatments. Scale bars, 100um. d Quantification of apoptotic rates based on TUNEL staining (n = 5). e RT-PCR analysis of IL-1β, IL-6 and TNFα in H9C2 cells transfected with vector or PIAS1 under normoxic condition or H/R treatment (n = 3). f H9C2 cells were transfected with PIAS1 plasmids as indicated; then, cells were cultured under normoxic or H/R conditions. Cells lysates were blotted with anti-PIAS1, anti- p-IκBα or anti-GAPDH antibody. g Quantification of the densitometry of the p-IκBα band in F (n = 3). Experiments in a, b, e, f and g were performed three times, and experiments in c and d were performed five times. Data presented are means ± SD, ##P < 0.01 vs. H/R + vector group, and **P < 0.01 vs. sham group

Back to article page