Table 1 Sense and reverse primers
From: PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPARγ SUMOylation
Genes | Species | F/R | Primers |
|---|---|---|---|
PIAS1 | Mouse | F | 5’-GCGGACAGTGCGGAACTAAA-3’ |
R | 5’-ATGCAGGGCTTTTGTAAGAAGT-3’ | ||
PIAS2 | Mouse | F | 5’-TTCCCTTATTCCAGTTGATCCCC-3’ |
R | 5’-CCACTGCTGGTTATGACCCC-3’ | ||
PIAS3 | Mouse | F | 5’-CAGCTCAGATTCTGTCTCTGTG-3’ |
R | 5’-TTCTTGGTTGGAGGGAGGTAA-3’ | ||
PIASy | Mouse | F | 5’-GGAGGCCAAAAACATGGTGAT-3’ |
R | 5’-GGGCTACAGTCGAACTGCAC-3’ | ||
RanBP2 | Mouse | F | 5’-GCTGGCTGCATTGTGCTATC-3’ |
R | 5’-GTGGGCCATCGTTTCCAGG-3’ | ||
Pc2 | Mouse | F | 5’-AAGAAGCGGATACGCAAGGG-3’ |
R | 5’-GGAGGAGTCTTGAAGCCCAG-3’ | ||
GAPDH | Mouse | F | 5’-AGGTCGGTGTGAACGGATTTG-3’ |
R | 5’-TGTAGACCATGTAGTTGAGGTCA-3’ | ||
IL-1β | Rat | F | 5’-CTGTGTCTTTCCCGTGGACC-3’ |
R | 5’-CAGCTCATATGGGTCCGACA-3’ | ||
IL-6 | Rat | F | 5’-GAACAACGATGATGCACTTGCAG-3’ |
R | 5’-CCTTAGCCACTCCTTCTGTGAC-3’ | ||
TNF-α | Rat | F | 5’-CCAGTGTGGGAAGCTGTCTT-3’ |
R | 5’-AAGCAAAAGAGGAGGCAACA-3’ | ||
GAPDH | Rat | F | 5’-TGTGTCCGTCGTGGATCTGA-3’ |
R | 5’-TTGCTGTTGAAGTCGCAGGAG-3’ |