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Fig. 1 | BMC Cell Biology

Fig. 1

From: Kif17 phosphorylation regulates photoreceptor outer segment turnover

Fig. 1

Schematic of transgenic phospho-mutant KIF17 constructs. a The amino acid sequence of the C-terminus of KIF17 in various species is depicted. Homologous sequences are shown in color (green, blue, and red). The green conserved sequence has been shown to interact with the IFT-B complex [9], while the blue conserved NLS can regulate ciliary localization [8]. The conserved serine (red), mouse S1029/zebrafish S815, has been shown to be phosphorylated by CaMKII [16]. b For mammalian cell culture experiments (Additional file 1: Figures S1, S2), three different mCherry-tagged constructs of mouse KIF17 under control of the ubiquitous CMV promoter were generated: wild-type KIF17, phospho-mimetic KIF17(S1029D), and phospho-deficient KIF17(S1029A). c For zebrafish cone photoreceptor experiments, three different GFP-tagged constructs of zebrafish under control of the cone-specific transducin-α promoter (TaCP) were generated: wild-type Kif17, phospho-mimetic Kif17(S815D), and phospho-deficient Kif17(S815A)

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