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Fig. 3 | BMC Cell Biology

Fig. 3

From: Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia

Fig. 3

Cytoskeletal changes in cells of sexually and asexually produced embryos of Daphnia magna. Double immunolabelling of α-tubulin (green) as marker for microtubules, and Phalloidin (red) as marker for actin filaments, counterstaining of the chromatin with DAPI (blue). a α-tubulin and Phalloidin were found in sexually produced embryos and b in asexually produced embryos. An interphase and an anaphase (asterisk) are shown in (a) whereas an interphase and a metaphase (asterisk) are shown in (b). c In cells of diapausing embryos, polymerized microtubules were nearly absent. Only two small green dots (white arrows) are observable; double immunolabelling of α-tubulin (green) is overlaid with pericentriolar material marker γ-tubulin (pink), presumably corresponding with the centrosome localization. Although nucleus size remains constant, actin networks display a significantly reduced cytoplasm during diapause. d In asexual embryos no difference between cytoskeletal architecture was observed between stage I and stage II organisms (e) in resuscitated D. magna sexually produced embryos, cytoskeletal features reappear at stage III accompanied with the onset of cell division. Polymerization of microtubules is visible and actin networks increase in complexity indicating cytoplasmatic growth. f Cytoskeleton is continuously expressed in stage III asexually produced embryos. Scale bars: 10 μm

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