Fig. 4

The changes of cytoskeleton in cells of sexually produced embryos of Daphnia magna over time at two temperatures. a Well-developed cytoskeleton nets were found in cells of young ephippia (about 74 h post ovulation). b After 18-day diapause, the cytoskeleton was still detectable, irrespective of temperature conditions at 20 °C and 4 °C. c Irrespective of temperature, the microtubule-nets were significantly reduced in 25-day diapausing embryos. Only some microtubular residues were still detectable. Actin networks were expanded. d After 30-days of diapause, microtubules were reduced to the centrosome and actin-networks were also reduced in their expansion. e After 90-day, all cytoskeletal elements (microtubules and actin microfilaments) were reduced to a minimum. Scale bars: 10 μm