Fig. 2

Blt1p and Gef2p function together in localization of Sid2p-mEGFP to the division site. Time shown in minutes; time zero represents SPB separation. a Time series of fluorescence micrographs at 6 min intervals in wild type (blt1⁺/gef2⁺, top row), blt1∆ (second row), gef2∆ (third row), or blt1∆/gef2∆ (bottom row) cells expressing Sid2p-mEGFP (black). Scale bar = 3 μm. b Time course of Sid2p localization to the division site in wild type cells (black line, ; n = 37), blt1∆ cells (blue line, ; n = 43), gef2∆ cells (green line, ; n = 35); or blt1∆/gef2∆ cells (gray line, Δ; n = 31). Error bars represent ±1 SD. c Time course of the mean number of molecules of Sid2p localized at the division site in wild type (black line ; n = 37), blt1∆ (blue line ; n = 43), gef2∆ (green line, ; n = 35), or blt1∆/gef2∆ (gray line, Δ; n = 31) cells. Error bars represent ±1 SD. Asterisks indicate time points at which the mean values of wildtype and mutant cells (blt1∆, gef2∆, blt1∆/gef2∆) differed with p < 0.0001