Fig. 4

Onset of contractile ring constriction and septation are delayed in blt1∆, gef2∆, and blt1∆/gef2∆ mutants. Time shown in minutes; time zero represents SPB separation. a Time series of fluorescence micrographs at 6 min intervals in wild type (blt1⁺/gef2⁺, top row), blt1∆ (second row), gef2∆ (third row), or blt1∆/gef2∆ (bottom row) cells expressing Rlc1p-mYFP (black). Scale bar = 3 μm. b Time course of the completion of Rlc1p nodes coalescence into the contractile ring in wildtype (black line, ; n = 92), blt1∆ cells (blue line, ; n = 107), gef2∆ cells (green line, ; n = 69) or blt1∆/gef2∆ cells (gray line, Δ; n = 121). Error bars represent ±1 SD. c Time course of the onset of contractile ring constriction in wildtype cells (black line, ; n = 87), blt1∆ cells (blue line, ; n = 96), gef2∆ cells (green line, ; n = 63) or blt1∆/gef2∆ cells (gray line, Δ; n = 112). Asterisks indicate time points at which the mean values of wildtype and mutant cells (blt1∆, gef2∆, blt1∆/gef2∆) differed with p < 0.0001. Error bars represent ±1 SD. d Mean duration of time to completion of contractile ring constriction in wildtype (wt, black) and mutant cells (blt1∆, blue; gef2∆, green; blt1∆/gef2∆, gray). Error bars represent ±1 SD. e Time course of septation completion resulting in two independent cells in wildtype (black line, ; n = 41), blt1∆ (blue line, ; n = 60), gef2∆ (green line, ; n = 37); or blt1∆/gef2∆ cells (gray line, Δ; n = 75). Error bars represent ±1 SD. Asterisks indicate time points at which the mean values of wildtype and mutant cells (blt1∆, gef2∆, blt1∆/gef2∆) differed with p < 0.0001