Fig. 5

mEGFP-Gef2p localization to the division site is disrupted in blt1∆ mutants. Time shown in minutes; time zero represents SPB separation. a Time series of fluorescence micrographs at 6 min intervals in wild type (gef2⁺, top row) and gef2∆ (bottom row) cells expressing Blt1p-mEGFP (black). Scale bar = 3 μm. b Time course of the mean number of molecules of Blt1p localized at the division plane in wild type cells (black line, ; n = 30) and gef2∆ cells (grey line, ; n = 24). Error bars represent ±1 SD. c Time course of Blt1p localization to the division site in wild type cells (black line, ; n = 23) and gef2∆ cells (grey line, ; n = 24). Error bars represent ±1 SD. d Time series of fluorescence micrographs taken at 6 min intervals in wildtype (blt1⁺, top row) and blt1∆ (bottom row) cells expressing mEGFP-Gef2p (black). Scale bar = 3 μm. e Time course of the mean number of molecules of Gef2p localized at the division plane in wild type cells (black line, ; n = 22) and blt1∆ cells (grey line, ; n = 39). Error bars represent ±1 SD. f Time course of Gef2p localization to the division site in wild type cells (black line, ; n = 22) and blt1∆ cells (grey line, ; n = 37). Error bars represent ±1 SD. Asterisk indicates range at which the mean values of wildtype and blt1∆ mutant cells differ with p < 0.001.