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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Fig. 4

Analysis of KIT copy variations in single cell clones. a Variation of the percentage of KIT copy with the splice mutation single cell clones as determined by the Nla III assay. D, duroc (pig breed with wild-type KIT allele); WT, non-edited Yorkshire pig kidney cells. b Variation of the percentage of the splice mutation in each single cell clone as reflected by the sequencing chromatograms. Black arrows indicate the G > A splice mutation. c KIT copy number in each single cell clone derived from cells edited by sgRNA16–1 determined by qPCR. (T-test, p < 0.05) (d) KIT copy number in each single cell clone derived from cells edited by sgRNA17–6 determined by qPCR. (T-test, p < 0.05) (e) Sequence analysis of cloned PCR products. DNA sequences of the wild-type (WT) and mutant clones, with CRISPR/Cas9 recognition sites shown in red and PAM sequences in blue. Dashes indicate deleted bases

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