Fig. 2

Experimental design and COLO 320DM-donor cells. a Graphical summary of the experimental design of this study. b–d Metaphase spreads from COLO 320DM-donor cells were hybridised with various probes. b Detection of IR/MAR plasmid sequences on all DMs in the COLO 320DM-donor cells. c, d Detection of human Alu (c, d) and c-myc sequences in the DMs in the COLO 320DM-donor cells (d). Most of the DMs were devoid of centromeric alphoid DNA (white arrows in c), although it was detected in a few DMs (yellow arrow in c). c-d Scale bar: 10 μm. e Frequencies of double-resistant colonies of monoclonal or polyclonal blasticidine-resistant human cell lines fused with neomycin-resistant mouse MEF acceptor cells. Colony number was calculated by examining three independent dishes containing 5 × 105 acceptor cells at the time of fusion. Mean +/− S.D. were calculated and are shown. Shown is a result from single experiment