Fig. 5

Various human DMs remain in the hybrid cells after pulverisation of human chromosomes. a-d Simultaneous detection of Alu and plasmid sequences (a, b) or detection of plasmid sequence (c, d) 4 weeks after fusion of COLO 320DM-donor cells with MEF (a), CHO-K1 (b), HeLa (c), or L929 (d) acceptor cells. DNA was counterstained by DAPI. DMs were identified by Alu-staining (a, b) or DAPI staining (c, d). Yellow and red arrows indicate DMs with and without the plasmid sequence, respectively. Scale bars: 10 μm. e Numbers of DMs with or without plasmid per hybrid cell. The box plot indicates the maximum and minimum (the right and the left end of the whiskers, respectively), first and third quartiles (both ends of the box), and median (line in the box). These values were obtained by examining 58 (MEF), 75 (CHO K1), 20 (HeLa), and 30 (L929) hybrid cells. Shown is a result from single experiment