Fig. 7
Microarray analysis of cloned hybrid cells reveals de novo generation of DMs from novel amplicons bearing tumorigenic genes. Hybrids of COLO 320DM-donor and MEF-acceptor cells were selected and grown under hypoxic (3% O2) conditions. From that culture, 24 clonal cell lines were obtained. Clones 4 (a) and 11 (b) had only c-myc-negative/Alu-positive DMs, whereas clone 19 (c) had both c-myc-positive and negative/Alu-positive DMs. The DNA from these cells, as well as DNA from COLO 320DM-donor and MEF-acceptor cells, were analysed by CytoScan™ HD Array; and the copy numbers per diploid cell for each cytoband were obtained. The copy number for the DNA from COLO 320DM-donor cells was standardised using data from normal human genomic DNA, and the copy number for the DNA from each clone was standardised using data from MEF-acceptor cells DNA. Panel d shows the cytobands that were amplified in at least one sample. Significantly amplified regions are boxed. 8q24.21 was amplified in all samples, but two different amplicons were detected (* and **). This is evident in panel e, where the copy numbers obtained for each gene within 8q24.21 are summarised. Shown is a result from single experiment