Fig. 1

Patronin-GFP localizes to a subset of the MT bundles that compose the mitotic spindle of S2 cells. Cells expressing Patronin-GFP and Cherry-tubulin were fixed and stained with anti-GFP (green) and anti-α-tubulin (red) antibodies, and with DAPI to detect DNA (blue). Prometa, prometaphase; Meta, metaphase; Ana, anaphase; Telo, telophase. Note that only a subset of the MT bundles associate with Patronin-GFP. In prometaphase, metaphase and early anaphase cells, the extremities of the Patronin-stained bundles are mostly excluded from the spindles poles (arrows in the metaphase cell), whereas in prophase and late telophase figures some bundles end at the center of the asters (arrowheads). Notably, in early anaphase cells the thin MTs bundles at the center of the cell (delimited by two arrows) are not enriched in Patronin. In late anaphase and telophase cells, the middle region of the central spindle, where the plus ends of antiparallel MTs overlap, is not associated with Patronin (asterisk). The white scale bar (5 μm) refers to all cells except telophases (red scale bar, 4 μm)