Fig. 3

Patronin exhibits dynamic behavior during prometaphase in S2 cells. Stills from a time-lapse video of an S2 cell prometaphase expressing Patronin-GFP. The numbers at the top of each frame indicate the time (min:sec) elapsed from the beginning of imaging. Note that Patronin-GFP seeds appear near the chromosomes (non-fluorescent dark spots) and extend towards the spindle poles along preexisting MT bundles, revealing an unexpected dynamic behavior of Patronin. See Additional file 3: Movie S1. Scale bar, 5 μm