Table 1 Frequencies of mitotic figures observed after RNAi against the indicated genes. To determine the frequencies of cells with abnormal centrosome numbers, we examined all mitotic cells. The frequencies of the different mitotic figures were instead determined by examining only cells with two centrosomes. Thus, the total numbers of cells examined in the two types of analyses are different. Prometa, prometaphases; Meta, metaphases; Ana, anaphases; Telo, telophases; PMLES, prometaphase-like cells with elongated spindles
RNAi | No. of cells | No. of centrosomes | No. of cells | Prometa (%) | Meta (%) | Ana (%) | Telo (%) | PMLES (%) | Ana+Telo (%) | |
|---|---|---|---|---|---|---|---|---|---|---|
1 cent. (%) | > 2 cent. (%) | |||||||||
none (Control) | 614 | 2.1 | 4.6 | 573 | 38.7 | 16.4 | 11.5 | 28.3 | 5.1 | 39.8 |
Patronin | 430 | 5.6** | 16.3** | 336 | 37.5 | 17.9 | 8.6 | 28.9 | 7.1 | 37.5 |
Klp10A | 235 | 4.3 | 9.4** | 203 | 31.0 | 16.7 | 7.9 | 33.5 | 10.8** | 41.4 |
Patronin + Klp10A | 251 | 1.2 | 6.8 | 231 | 42.0 | 19.5 | 4.8** | 23.4 | 10.4** | 28.1**§ |
asp | 273 | 5.9** | 3.3 | 248 | 34.3 | 23.0 | 7.3 | 23.4 | 12.1** | 30.6* |
Patronin + asp | 272 | 7.4** | 22.4** | 191 | 39.8 | 26.2 | 1.0** | 11.5** | 21.5** | 12.6**§ |
dgt6 | 303 | 5.9** | 4.0 | 273 | 45.4 | 19.0 | 2.6** | 9.2** | 23.8** | 11.7** |
Patronin + dgt6 | 260 | 2.3 | 13.1** | 220 | 45.0 | 18.2 | 5.5* | 19.5* | 11.8** | 25.0** |