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Table 2 Forward (F) and reverse (R) primer sequence details used in the current study

From: Genes involved in mitochondrial biogenesis and function may not show synchronised responses to mitochondria in shell gland of laying chickens under infectious bronchitis virus challenge

Gene name

Abbreviation used

Primer sequence (5′ – 3′)

Amplicon size (bp)

Annealing temperature °C

Amplification efficiency (%)

R2

Slope

Accession No.

Glyceraldehyde-3-phosphate dehydrogenase

GAPDH a

F-GGTCACCAAGAAGGTGGAGA

R-GACAGTGCCCTTGAAGTGTC

137

63

96

0.99888

−3.434

NC_006088.3

NADH dehydrogenase subunit 4

ND4 b

F-CGCAGGCTCCATACTACTCG

R-TTAGGGCACCTCATAGGGCT

137

60

99

0.99944

−3.341

NC_001323.1

Citrate synthase

CS

F-TACTACACGGTGCTCTTCGG

R-CGGATCCTGCCGGATTTGTAG

152

60

96

0.99943

−3.089

XM_015300287.1

Succinate dehydrogenase complex flavoprotein subunit A

SDHA

F-TCTGTCCATGGTGCTAATCG

R-TGGTTTAATGGAGGGGACTG

126

60

94

0.99790

−3.484

NM_001277398.1

Dynamin-1-like protein

Drp1/DNM1L

F-TGTGACCCGAAGACCCCTTA

R-AGCATCTATCTCATTTTCATCTCCA

91

60

99

0.99861

−3.345

NM_001079722.1

Cytochrome C, somatic

CYC, S

F-CCCAGTGCCATACGGTTGAA

R-CTCACCCCAAGTGATACCTTTGT

140

60

96

0.9930

−3.430

NM_001079478.1

PPARG coactivator 1 alpha

PGC-1α/PPARGC1A

F-AGTGACATCGAGTGTGCTGCT

R-GGTCAAGTTCTGGGAGATCTGGG

70

63

100

0.99472

−3.331

NM_001006457.1

(Na+-K+) ATPase

(Na + -K + ) ATPase *

F-GTCAACCCGAGGGATGCTAA

R-ACTGCTACAATGGCACCCTG

179

60

99

0.99663

−3.338

J03230.1

S1 glycoprotein

IBV T

F-TCAGGTGGTTGGCATTTACA

R-ATTGCGAACTTGACCATTCC

181

60

99

0.99982

−3.323

U29522.1

Nucleocapsid protein (3′ UTR)

IBV Vic S

F-ATAGGCATGTAGCTTGATTACC

R-GTTTCCAGGCTACTAAGTAGAC

76

60

98

0.99823

−3.320

DQ059623.1

TATA-Box Binding Protein

TBP*

F-TAGCCCGATGATGCCGTAT

R-GTTCCCTGTGTCGCTTGC

147

61

97

0.99676

−3.407

NM_205103

Tyrosine 3-monooxygenase/tryptophan

5-monooxygenase activation protein, zeta polypeptide

YWHAZ*

F- TTGCTGCTGGAGATGACAAG

R- CTTCTTGATACGCCTGTTG

60

60

104

0.99912

−3.222

NM_001031343.1

  1. aGene was used to amplify fragment of genomic DNA; bGene was used to amplify fragment of mtDNA
  2. *The primer sequences for IBV Vic S [69], TBP [86], YWHAZ [87] and (Na+-K+) ATPase [88] were sourced from literature