Gene name | Abbreviation used | Primer sequence (5′ – 3′) | Amplicon size (bp) | Annealing temperature °C | Amplification efficiency (%) | R2 | Slope | Accession No. |
---|---|---|---|---|---|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH a | F-GGTCACCAAGAAGGTGGAGA R-GACAGTGCCCTTGAAGTGTC | 137 | 63 | 96 | 0.99888 | −3.434 | NC_006088.3 |
NADH dehydrogenase subunit 4 | ND4 b | F-CGCAGGCTCCATACTACTCG R-TTAGGGCACCTCATAGGGCT | 137 | 60 | 99 | 0.99944 | −3.341 | NC_001323.1 |
Citrate synthase | CS | F-TACTACACGGTGCTCTTCGG R-CGGATCCTGCCGGATTTGTAG | 152 | 60 | 96 | 0.99943 | −3.089 | XM_015300287.1 |
Succinate dehydrogenase complex flavoprotein subunit A | SDHA | F-TCTGTCCATGGTGCTAATCG R-TGGTTTAATGGAGGGGACTG | 126 | 60 | 94 | 0.99790 | −3.484 | NM_001277398.1 |
Dynamin-1-like protein | Drp1/DNM1L | F-TGTGACCCGAAGACCCCTTA R-AGCATCTATCTCATTTTCATCTCCA | 91 | 60 | 99 | 0.99861 | −3.345 | NM_001079722.1 |
Cytochrome C, somatic | CYC, S | F-CCCAGTGCCATACGGTTGAA R-CTCACCCCAAGTGATACCTTTGT | 140 | 60 | 96 | 0.9930 | −3.430 | NM_001079478.1 |
PPARG coactivator 1 alpha | PGC-1α/PPARGC1A | F-AGTGACATCGAGTGTGCTGCT R-GGTCAAGTTCTGGGAGATCTGGG | 70 | 63 | 100 | 0.99472 | −3.331 | NM_001006457.1 |
(Na+-K+) ATPase | (Na + -K + ) ATPase * | F-GTCAACCCGAGGGATGCTAA R-ACTGCTACAATGGCACCCTG | 179 | 60 | 99 | 0.99663 | −3.338 | J03230.1 |
S1 glycoprotein | IBV T | F-TCAGGTGGTTGGCATTTACA R-ATTGCGAACTTGACCATTCC | 181 | 60 | 99 | 0.99982 | −3.323 | U29522.1 |
Nucleocapsid protein (3′ UTR) | IBV Vic S | F-ATAGGCATGTAGCTTGATTACC R-GTTTCCAGGCTACTAAGTAGAC | 76 | 60 | 98 | 0.99823 | −3.320 | DQ059623.1 |
TATA-Box Binding Protein | TBP* | F-TAGCCCGATGATGCCGTAT R-GTTCCCTGTGTCGCTTGC | 147 | 61 | 97 | 0.99676 | −3.407 | NM_205103 |
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | YWHAZ* | F- TTGCTGCTGGAGATGACAAG R- CTTCTTGATACGCCTGTTG | 60 | 60 | 104 | 0.99912 | −3.222 | NM_001031343.1 |