Skip to main content
Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus

Fig. 2

Lamin A/C depletion mislocalizes nuclear myosin I (NM1) in an Emerin dependent manner. a Immunostaining for NM1 (green) and Emerin (red) in control (untreated, siLacZ), single and combined knockdowns of Lamin A/C and Emerin. Scale bar ~ 10 μm. Inset: Zoomed in images b Single representative nuclei showing intranuclear NM1 foci (green) and Emerin (red) in control (untreated, siLacZ) cells, single and co-depletion of Lamin A/C and Emerin. Scale bar ~ 10 μm c Mean % frequency of cells (from a, b) showing aggregates of NM1 in the cytoplasm. Error bars: SEM, N = 3, ***p < 0.0001 d Dot scatter plot showing number of intranuclear NM1 foci (mid-optical section) in control (untreated, siLacZ) cells, single and co-depletion of Lamin A/C and Emerin. Horizontal lines: Median (M), n = number of nuclei analysed, N = 2, *** p < 0.0001 (N: number of independent biological replicates) e Representative Co-IP using anti-Emerin antibody, probed for NM1 and Lamin A in control (untreated) and Lamin A/C depleted cells (48 h post Lamin A/C Kd, N = 3) f Co-IP performed on whole cell extracts (WCE) and nuclear extracts (NE) of DLD-1 cells using anti-NM1 antibody and probed for actin (N = 2)

Back to article page