Fig. 6

Lamin A/C-Emerin co-depletion enhances histone mobility at the nuclear interior. a Representative FRAP images for H2A-mCherry in control (siLacZ) cells, and upon single and co-depletion of Lamin A/C and Emerin. Scale bar ~ 10 μm. b (left) Mean % mobile fractions for internal and peripheral ROIs for H2A-mCherry in Control (siLac Z cells), single and co-depletion of Lamin A/C and Emerin. Data shown is a compilation from N = 3, 3–6 nuclei per replicate, Error bars = SEM, *p < 0.05, (right) Zoomed in image shows ROI (internal and peripheral) used for FRAP. c-d FRAP recovery curves for (c) peripheral and (d) internal ROIs for H2A-mCherry in control (siLacZ cells), single and co-depletion of Lamin A/C and Emerin. NFI = normalized fluorescence intensity, c (ii) and d (ii) rescaled graphs showing differences in recovery patterns between peripheral and internal ROIs. Data shown is representative experiment out of N = 3, 3–6 nuclei per replicate, Error bars: SEM. (N: number of independent biological replicates)