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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus

Fig. 7

Actin depolymerization and NM1 inhibition in Lamin A/C-Emerin co-depleted cells restores chromatin dynamics. a FRAP images of H2A-mCherry in control (siLacZ) and siLamin A/C + siEmerin cells with DMSO (control) or Latrunculin A (50 nM) treatment. Scale bar ~ 10 μm. b Mean % mobile fractions for Internal and Peripheral ROIs for H2A-mCherry in Control (siLacZ) and siLamin A/C + siEmerin DLD-1 cells with DMSO or Latrunculin A (50 nM) treatment. Error bars: SEM, N = 3, ~ 6–12 nuclei per category, *p < 0.05. c FRAP images of H2A-mCherry in control (siLacZ) and siLamin A/C + siEmerin cells with NFW (control: nuclease free water) or BDM (1 mM) treatment. Scale bar ~ 10 μm. d Mean % mobile fractions for Internal and Peripheral ROIs for H2A-mCherry in Control (siLacZ) and siLamin A/C + siEmerin DLD-1 cells with NFW or BDM (1 mM) treatment. Error bars: SEM, N = 2, ~ 5–8 nuclei per category, *p < 0.05

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