Fig. 1

Selection and validation of predicted miRNAs binding to the mouse Ndrg2 3’UTR. a Venn diagram of miRNAs predicted from miRWalk2.0 and microRNA.org algorithms, and selection criteria used to identify potential miRNAs binding Ndrg2 3’UTR. b Nucleotide sequence alignment between the seed sites in the Ndrg2 3’UTR and predicted miRNAs with their corresponding mirSVR scores. c Dual-luciferase reporter assays determining the interaction of overexpressed miR-23a (dark grey bar), -23b (light grey bar), -28 (white bar), -181a (striped bar) and negative control (NC, black bar) with full-length Ndrg2 3’UTR; (d), with the miR-23a/−23b seed site (miR23seed) or its mutated version (miR23mut); and (e), with the miR-28 seed site (miR28seed) or its mutated version (miR28mut). Data is representative of three independent experiments with n = 5–6 per sample group, and expression levels are presented as arbitrary units (AU). **p < 0.01 and ****p < 0.0001 to NC