Fig. 5

The effect of combined miRNA overexpression on endogenous NDRG2 mRNA and protein levels under basal and catabolic conditions. miR-23a, -23b and -28 were co-transfected into myotubes and their combined effects on NDRG2 regulation was measured in the presence or absence of 48 h treatment with 10 μM dexamethasone. a Ndrg2 mRNA and (b) NDRG2 protein levels under basal conditions following miRNA co-treatment (striped bar) or negative control miRNA (NC, black bar) transfection. c Ndrg2 mRNA and (d) NDRG2 protein levels following the combined effects of DMSO vehicle and negative control miRNA treatment (Veh + NC; grey bar), dexamethasone and negative control miRNA treatment (Dex + NC; stippled bar), or dexamethasone and miRNA mix treatment (Dex + MM; cross-hatched bar). Data are representative of two independent experiments with n = 4 per sample group, and expression levels are presented as arbitrary units (AU). *p < 0.05, **p < 0.01 or ***p < 0.001 to Dex + NC treatment