Fig. 1

Drosophila tbc1 is highly conserved and is expressed in Drosophila embryos a A Phylip unrooted tree analysis of Tbc1 orthologues. Asterisks indicate species included in the alignment. b Alignment of a subset the Tbc1 orthologues generated with tools available at the CLC Workbench (Qiagen). Amino acids are colored along a gradient by positional percent conservation across species. Conservation is displayed as a bar chart below the alignment. Blue shading is poor conservation (dark blue 0% conservation), whereas red shading is high conservation (dark red 100% conservation). Geen bar above sequence indicates the TBC domain; purple bar above sequence indicates the Rhodanese-like domain. c Whole-mount in situs with probes detecting tbc1 mRNA in wild-type embryos. Black arrows, SG; white arrows, proventriculus; arrowhead, hindgut. In stage 16, there is also hindgut expression that is not visible in the focal plane shown. The Phylip unrooted tree and alignments were constructed with the tools available at [https://www.genome.jp/toolsbin/clustalw]