Fig. 1

Results of ChIP and EMSA demonstrate that FOXL2 is regulated by STAT3. a ChIP demonstrates that anti-STAT3 antibodies immunoprecipitate FOXL2. The FOXL2 gene is detected in nuclear protein (stimulating with human IL-6) immunoprecipitated with anti-STAT3 antibody using PCR (down), and the detected primers used in the PCR are designed as shown (above), demonstrating that STAT3 binds to the FOXL2 promoter which contains the predicted sites. b EMSA results using biotin-labeled and unlabeled probes that contain STAT3 predicted binding sites, show that nuclear proteins bind to the biotinylated DNA fragments and that the addition of the corresponding cold DNA fragment (unlabeled probes) or anti-STAT3 antibody attenuates this binding