Fig. 2

Transfection with STAT3 siRNA inhibits the expression of phosphorylated-STAT3 and FOXL2. a Lipofectamine 3000 and the Red Fluorescent Control complexes (doses range from 0 to 50 nM) indicate that HeLa cells have relatively higher transfection efficiencies at 30 nM. b, c qRT-PCR demonstrated that STAT3 siRNA downregulates the mRNA levels of phosphorylated STAT3 and FOXL2 relative to GAPDH. The cells with no transfection or transfected with negative siRNA (Thermo Fisher) were both used as controls. Each column represents the mean ± SEM of at least three replicates, and the whole experiment was repeated 3 times, * and ** indicate significant differences at P < 0.05 or P < 0.01, respectively. d Western blotting demonstrates that STAT3 siRNA reduces p-STAT3 and FOXL2 protein levels in a dose dependent manner, whereas the control and negative siRNA show almost no reduction