Fig. 1

Age-dependent expression of Cited 2 in mice heart tissues and CSCs. a. RT-PCR for Cited2 mRNA expression in Young (2–3 months), Middle (4–6 months) and Old (20–22 months) mice heart tissues. b. The analysis of RT-PCR for Cited2 mRNA expression. c. DD-PCR for Cited2 mRNA expression in Young (2–3 months) and Old (20–22 months) CSCs. d-g. Immunoblotting of CITED2 protein levels in lentivector transduction groups and CSCs groups. OE represented Old CSCs transducted with lentiviral particles of Cited2-cDNA, KD represented Young CSCs transducted with lentiviral particles of Cited2-shRNA3, the control was transducted with empty lentivector. Data were from three independent experiments and presented as mean ± SD. Statistical analysis was implemented by GraphPad Prism 5, *p < 0.05, ** p < 0.01, and *** p < 0.001, Old-CSCs contrast to Young-CSCs