Fig. 6
From: Cited2 regulates proliferation and survival in young and old mouse cardiac stem cells
Cited2 affects cell apoptosis with CSCs aging. The apoptosis was tested by FCM with Annexin V-PE apoptosis detection kit and the PE-positive cells percentage stand for the ratio of apoptosis cells to all numbered cells. a The apoptosis rate in Old CSCs transducted with lentiviral particles of Cited2-cDNA (left panel) and empty lentivector (right panel). b Statistical analysis of apoptosis rate was implemented by GraphPad Prism 5. c The apoptosis rate in Young CSCs transducted with lentiviral particles of Cited2-shRNA3 (left panel) and empty lentivector(right panel). d Statistical analysis of apoptosis rate was implemented by GraphPad Prism 5. *p < 0.05, ** p < 0.01, and *** p < 0.001, contrast to control. Data are based on three independent experiments and the picture represents a single result. Overexpression represented Old CSCs transducted with lentiviral particles of Cited2-cDNA. Knockdown represented Young CSCs transducted with lentiviral particles of Cited2-shRNA3.The negative control was transducted with empty lentivector