Table 1 Sequences of Cited2-shRNA and the primers
From: Cited2 regulates proliferation and survival in young and old mouse cardiac stem cells
Primer | Forward primer 5′ → 3′ | Reverse primer 5′ → 3′ |
|---|---|---|
Cited2 (for dd PCR and RT-PCR) | AAATCGCAAAGACGGAAGGA | TGAACTGGGAGTTGTTAAACCT |
Cited2-shRNA1 | TGGCAGACCATATGATGGCCATGAA | Â |
Cited2-shRNA2 | CCCACAACCACTACATGCCGGATTT | Â |
Cited2-shRNA3 | GTCAGCTGTTGACTCGGTTAA | Â |
Cited2-cDNA | tatcgaattc(EcoRI)ATGGCAGACCATATGATGGCCATGAAC | tatcggatcc(BamHI)ACAGCTGACTCTGCTGGGCTGCT |
GAPDH | GCTGCCCAGAACATCATCCCT | TGAAGTCGCAGGAGACAACC |