Fig. 4From: Alternative splicing of the Wnt trafficking protein, Wntless and its effects on protein-protein interactionsGST-pulldown and mapping of WlsX/GPM6 interaction. Western blot (top panel) was probed with an HRP-conjugated S-tag antibody. A small sample from each of the GST pulldowns was run on a separate gel for coomassie stain and pulldown verification. The second lane represents a control pulldown in which the beads were coated in GST onlyBack to article page