Fig. 3

High glucose affects the protein expression of apoptosis related, Stat1 and FoxO1 in MPC-5 cells. The experiments were divided into three groups, which were control (5.5 mmol/L normal glucose), NM (5.5 mmol/L glucose+ 24.5 mmol/L mannitol) and HG (30 mmol/L glucose). a The cleaved caspased-3, Bcl-2, Bax, Stat1 and FoxO1 protein levels were detected by western blot in MPC-5 cells. b Relative protein expression of cleaved caspased-3 showed as bar diagrams. c Relative protein expression of Bcl-2 showed as bar diagrams. d Relative protein expression of Bax showed as bar diagrams. e Relative protein expression of Stat1 showed as bar diagrams. f Relative protein expression of FoxO1 showed as bar diagrams. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, *P < 0.05, **P < 0.01)