Fig. 5

Silencing Stat1affects the protein expression of apoptosis related, Stat1 and FoxO1 in MPC-5 cells. The experiments were divided into five groups, which were control, HG (30 μM), NC + HG (Negative siRNA + 30 μM HG), siStat1 + HG (Stat1 siRNA + 30 μM HG) and SiStat1 (Stat1 siRNA). a The cleaved caspased-3, Bcl-2, Bax, Stat1 and FoxO1 protein levels were detected by western blot in MPC-5 cells. b Relative protein expression of cleaved caspased-3 showed as bar diagrams. c Relative protein expression of Bcl-2 showed as bar diagrams. d Relative protein expression of Bax showed as bar diagrams. e Relative protein expression of Stat1 showed as bar diagrams. F Relative protein expression of FoxO1 showed as bar diagrams. GAPDH served as an internal control. g The protein expressions of FoxO1 in nucleus and cytoplasm, respectively, Lamin B1 served as a nucleus loading control, GAPDH served as a cytoplasm internal control. h Relative protein expression of FoxO1 in nucleus showed as bar diagrams. i Relative protein expression of FoxO1 in cytoplasm showed as bar diagrams. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, ^#Compared with HG, */^#P < 0.05, **/^##P < 0.01)