Fig. 9

The effect of over-expressing FoxO1 on the expression of antioxidant genes, Stat1 and FoxO1. The experiments were divided into three groups, which were control, Mock + HG and over-expressing FoxO1 + HG. a The protein expression of FoxO1 in nucleus and cytoplasm, respectively, Lamin B1 served as a nucleus loading control, Tubulin served as a cytoplasm internal control. b Relative protein expressions of FoxO1 in nucleus and cytoplasm showed as bar diagrams. c The Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 protein levels were detected by western blot in MPC-5 cells. d Relative protein expression of Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 showed as bar diagrams. e mRNA expression of Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 were assessed using qRT-PCR in MPC-5 cells. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, ^# Compared with Mock + HG, */^#P < 0.05, **/^##P < 0.01)