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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies

Fig. 3

Identification of functionality and purity of endothelial cells. a) Isolated cells were identified as endothelial cells by CD31 (PECAM-1) antibody (MCA1746GA, AbD Serotec) immunostaining (passage 3). b) One of the characteristics of endothelial cells is tube formation. Functional analysis of the cells was performed by using an angiogenesis assay (Matrigel™ GFR Membrane Matrix, #356231, Corning) (passage 2). c) Purity of the cells was ensured by using fluorescence assisted cell sorting (FACS) resulting in purity of 97.81%, which can be considered as a pure preparation of ECs (passage 2)

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