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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

Fig. 7

Strand-displacement activity of polymerase I large fragment from Geobacillus stearothermophilus (Gbst pol I LF) and Ureibacillus thermosphaericus (Ubts pol I LF) (wild type, WT, blue) and their respective D422A variants (red). Time resolved strand-displacement assays were performed at 37 °C in 20 mM Tris pH 7.9 (at 25 °C), 100 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 0.1% Triton X-100. The increase in TAMRA fluorescence, i.e. enzyme activity, has been measured as relative fluorescence units (RFUs). Fifty nanogram Gbst pol I LF and 100 ng Ubts pol I LF were used in the reaction setup

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