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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Temporal integration of mitochondrial stress signals by the PINK1:Parkin pathway

Fig. 3

Fluctuations in mitochondrial membrane potential drive transient PINK1 stabilization in cells incubated with low doses of CCCP. a time-lapse images of PINK1-EGFP (green) and TMRM (red) fluorescence in a HeLa cell incubated with 5 μM CCCP. Partial recovery of mitochondrial membrane polarization at t = 90 min (as indicated by increased TMRM fluorescence) is followed by a decrease in PINK1-EGFP levels. b quantification of PINK1-EGFP and TMRM fluorescence in single live HeLa cells incubated in 5 μM CCCP. In each of the 4 representative cells, increased TMRM fluorescence was associated with rapid loss of PINK1-EGFP. Scale bar represents 50 μm

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