Fig. 8

The PINK1 response to cycles of de- and repolarization of the mitochondria is stereotyped. a-d HeLa cells expressing PINK1-EGFP were exposed to 60 min pulses of 10 μM CCCP every 100 min, as depicted in the schematic (a), and imaged by live cell fluorescence microscopy. b population average trajectories of PINK1-EGFP fluorescence based on 3 biological repeats and a total of 19 cells. Error is represented as the S.E. c trajectories of PINK1-EGFP fluorescence for 4 representative cells. d, peak and trough analysis of PINK1-EGFP levels. Error is represented as the S.E. Statistical significance was determined by one-way ANOVA. e HeLa cells were treated with 60 min pulses of 10 μM CCCP every 100 min, as represented in the schematic, harvested at the indicated time points and immunoblotted for PINK1 and actin as a loading control. f PINK1 levels at each timepoint were quantified by densitometry and normalized to actin. Statistical differences in PINK1 levels between samples were appraised by one-way ANOVA and Tukey’s multiple comparisons follow-up test. Statistical significance is indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Data is from 3 biological repeats