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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: NFE2/miR-423-5p/TFF1 axis regulates high glucose-induced apoptosis in retinal pigment epithelial cells

Fig. 1

MiR-423-5p is upregulated in DR and exacerbates high glucose-induced apoptosis in RPE cells. a The relative levels of miR-423-5p were measured by qRT-PCR in the normal group and DR group showed that miR-423-5p was upregulated in DR group. b The relative levels of miR-423-5p were measured by qRT-PCR in ARPE-19 and RPE-J cells treated with NG or HG. The data demonstrated that miR-423-5p was upregulated in HG-treated cells. For c-e, ARPE-19 and RPE-J cells were transfected with NC mimics, miR-423-5p mimics, NC inhibitor or miR-423-5p inhibitor. c qRT-PCR analysis of miR-423-5p in ARPE-19 and RPE-J cells after transfection with miR-423-5p mimics, NC mimics, miR-185-3p inhibitor, or NC inhibitor. Transfection with miR-423-5p mimics successfully boosted the expression of miR-423-5p, while transfection with miR-423-5p inhibitor reduced the expression of miR-423-5p. d Apoptosis rates of ARPE-19 and RPE-J cells were measured by Annexin V/PI staining and flow cytometry. The results suggested that overexpression of miR-423-5p resulted in a significant increase in HG-induced apoptosis, while HG-induced apoptosis was significantly repressed by silencing miR-423-5p. e The relative protein levels of cleaved-caspase 3, Bax and Bcl-2 were assessed by Western blotting, which indicated that transfection of the miR-423-5p mimic significantly upregulated the protein levels of cleaved-caspase 3 and Bax, and downregulated the level of Bcl-2. However, transfection of a miR-423-5p inhibitor led to opposite effect. Data are presented as mean ± SD. Three replicate wells were used per sample in each experiment and experiments were replicated three times. * P < 0.05; ** P < 0.01; *** P < 0.001

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