Fig. 3

Intrinsic emission and far ultraviolet CD spectra of tubulin dimers. a The groups of brain and sperm tubulins show a different intrinsic emission of tubulins. b There was no significant differences of emission spectra in the presence of ELEF. c The extreme modifications in secondary structure were observed in the presence of ELEF. d The changes of ellipticity are presented at 220 nm in sperm, and brain controls, at 221 nm in brain ELEF, and at 224 nm in sperm ELEF. The assigned letters of a, b, c, and d indicate significant differences (P < 0.05) among the groups. The result is from 3 independent experiments. Values are expressed as mean ± SD, Tukey post hoc test